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rabbit polyclonal anti ac p53  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti ac p53
    Rabbit Polyclonal Anti Ac P53, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 6423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+ac+p53/p53+Antibody/pmc11393428__41467_2024_52363_MOESM9_ESM-61-0-3
    Average 97 stars, based on 6423 article reviews
    rabbit polyclonal anti ac p53 - by Bioz Stars, 2026-09
    97/100 stars

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    Article Title: Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner
    Article Snippet: .. Rabbit polyclonal anti-ac-p53, Cell Signaling Technology, Cat 2525, dilution 1:1000 for western blotting. ..



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    Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and <t>p53.</t> (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ- HDAC9 and wild-type controls.
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    rabbit polyclonal anti ac p53  (Cell Signaling Technology Inc)
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    Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and <t>p53.</t> (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ- HDAC9 and wild-type controls.
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    ac p53  (OriGene)
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    Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and <t>p53.</t> (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ- HDAC9 and wild-type controls.
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    rabbit anti mouse ac p53 polyclonal antibody  (Santa Cruz Biotechnology)
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    Effects of STL on the protein expression of SIRT1, <t>Ac-p53,</t> Bcl-2 and Bax in the brain 24 hours after cerebral I/R. (A) Western blot assay for protein expression in each group. (B–E) Density analysis of SIRT1 (B), Ac-p53 (C), Bcl-2 (D), and Bax (E) protein expression. β-Actin was used as a loading control. The protein expressions were expressed as the optical density percentage of the target protein to β-actin. Values are expressed as the mean ± SEM ( n = 4 rats per group), and were analyzed by analysis of variance followed by the least significant difference test. ** P < 0.01, vs . sham group; ## P < 0.01, vs . I/R group; && P < 0.01, vs . I/R + STL (17.2 mL/kg) group. STL: Shuan-Tong-Ling ; I/R: ischemia/reperfusion; Ac-p53: acetylated-protein 53; EX527: SIRT1 inhibitor; SIRT1: silent information regulator 1.
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    rabbit polyclonal anti ac-p53  (Cell Signaling Technology Inc)
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    Effects of STL on the protein expression of SIRT1, <t>Ac-p53,</t> Bcl-2 and Bax in the brain 24 hours after cerebral I/R. (A) Western blot assay for protein expression in each group. (B–E) Density analysis of SIRT1 (B), Ac-p53 (C), Bcl-2 (D), and Bax (E) protein expression. β-Actin was used as a loading control. The protein expressions were expressed as the optical density percentage of the target protein to β-actin. Values are expressed as the mean ± SEM ( n = 4 rats per group), and were analyzed by analysis of variance followed by the least significant difference test. ** P < 0.01, vs . sham group; ## P < 0.01, vs . I/R group; && P < 0.01, vs . I/R + STL (17.2 mL/kg) group. STL: Shuan-Tong-Ling ; I/R: ischemia/reperfusion; Ac-p53: acetylated-protein 53; EX527: SIRT1 inhibitor; SIRT1: silent information regulator 1.
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    anti ac lys 382 p53 rabbit polyclonal  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology anti ac lys 382 p53 rabbit polyclonal
    FIGURE 1. Composition of transcriptional complexes associated with Ac-Lys-373 <t>p53</t> and Ac-Lys-382 p53. Nuclear fractions were prepared from LNCaPcellstreatedwithCG-1521andTSAfor8h.A,acetylationandphosphoryl- ationstatusofp53aftertreatmentwithCG-1521orTSA.B,real-timePCRanalysis of expression changes of p21 from LNCaP cells treated with CG-1521 and TSA from 0 to 72 h of treatment.
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    Image Search Results


    Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and p53. (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ- HDAC9 and wild-type controls.

    Journal: Disease Models & Mechanisms

    Article Title: Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice

    doi: 10.1242/dmm.023366

    Figure Lengend Snippet: Eµ-HDAC9 tumors display deregulated acetylation of BCL6 and p53. (A) IHC triple-immunostaining using the ABC-TSA method in mouse spleens from Eμ-HDAC9 and wild-type tumors, showing HDAC9 (red) expression in conjunction with levels of acetylated (Ac)-BCL6 (green). (B) Immunoblot analysis of Ac-p53 in Eµ- HDAC9 and wild-type spleen. GAPDH was used as a loading control. (C) ABC-TSA immunofluorescence analysis of Ac-p53 (green) and HDAC9 (red) in spleen in Eμ- HDAC9 and wild-type controls.

    Article Snippet: Primary antibodies included polyclonal rabbit anti-Ac-p53 (Lys379) (Thermo Scientific), monoclonal rat anti-HDAC9 antibody (clone 45a7b5b), monoclonal mouse anti-FLAG M2 (Sigma), polyclonal rabbit anti-acetylated lysine (Millipore 06-933), polyclonal rabbit anti-BCL6 (ab19011), anti-CD45R(B220) (ab64100) and anti-CD3 (ab5690).

    Techniques: Triple Immunostaining, Expressing, Western Blot, Control, Immunofluorescence

    Effects of STL on the protein expression of SIRT1, Ac-p53, Bcl-2 and Bax in the brain 24 hours after cerebral I/R. (A) Western blot assay for protein expression in each group. (B–E) Density analysis of SIRT1 (B), Ac-p53 (C), Bcl-2 (D), and Bax (E) protein expression. β-Actin was used as a loading control. The protein expressions were expressed as the optical density percentage of the target protein to β-actin. Values are expressed as the mean ± SEM ( n = 4 rats per group), and were analyzed by analysis of variance followed by the least significant difference test. ** P < 0.01, vs . sham group; ## P < 0.01, vs . I/R group; && P < 0.01, vs . I/R + STL (17.2 mL/kg) group. STL: Shuan-Tong-Ling ; I/R: ischemia/reperfusion; Ac-p53: acetylated-protein 53; EX527: SIRT1 inhibitor; SIRT1: silent information regulator 1.

    Journal: Neural Regeneration Research

    Article Title: Fermented Chinese formula Shuan-Tong-Ling attenuates ischemic stroke by inhibiting inflammation and apoptosis

    doi: 10.4103/1673-5374.202946

    Figure Lengend Snippet: Effects of STL on the protein expression of SIRT1, Ac-p53, Bcl-2 and Bax in the brain 24 hours after cerebral I/R. (A) Western blot assay for protein expression in each group. (B–E) Density analysis of SIRT1 (B), Ac-p53 (C), Bcl-2 (D), and Bax (E) protein expression. β-Actin was used as a loading control. The protein expressions were expressed as the optical density percentage of the target protein to β-actin. Values are expressed as the mean ± SEM ( n = 4 rats per group), and were analyzed by analysis of variance followed by the least significant difference test. ** P < 0.01, vs . sham group; ## P < 0.01, vs . I/R group; && P < 0.01, vs . I/R + STL (17.2 mL/kg) group. STL: Shuan-Tong-Ling ; I/R: ischemia/reperfusion; Ac-p53: acetylated-protein 53; EX527: SIRT1 inhibitor; SIRT1: silent information regulator 1.

    Article Snippet: The membranes were incubated overnight with rabbit anti-mouse SIRT1 polyclonal antibody (1:1,000; Abcam, Cambridge, MA, USA), rabbit anti-mouse Ac-p53 polyclonal antibody (1:500; Santa Cruz Biotechnology, Buffalo, CA, USA), anti-mouse Bcl-2 monoclonal antibody (1:500; Santa Cruz Biotechnology), anti-mouse Bax monoclonal antibody (1:500; Santa Cruz Biotechnology) and goat anti-rat β-actin antibody (1:2,000; Boster, Wuhan, Hubei Province, China) at 4°C.

    Techniques: Expressing, Western Blot, Control

    FIGURE 1. Composition of transcriptional complexes associated with Ac-Lys-373 p53 and Ac-Lys-382 p53. Nuclear fractions were prepared from LNCaPcellstreatedwithCG-1521andTSAfor8h.A,acetylationandphosphoryl- ationstatusofp53aftertreatmentwithCG-1521orTSA.B,real-timePCRanalysis of expression changes of p21 from LNCaP cells treated with CG-1521 and TSA from 0 to 72 h of treatment.

    Journal: Journal of Biological Chemistry

    Article Title: Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly

    doi: 10.1074/jbc.m609588200

    Figure Lengend Snippet: FIGURE 1. Composition of transcriptional complexes associated with Ac-Lys-373 p53 and Ac-Lys-382 p53. Nuclear fractions were prepared from LNCaPcellstreatedwithCG-1521andTSAfor8h.A,acetylationandphosphoryl- ationstatusofp53aftertreatmentwithCG-1521orTSA.B,real-timePCRanalysis of expression changes of p21 from LNCaP cells treated with CG-1521 and TSA from 0 to 72 h of treatment.

    Article Snippet: The antibodies used for the immunoprecipitation assay were: anti-total p53 mouse monoclonal (05-224; Upstate Biotechnology), antiAc-Lys-373 p53 rabbit polyclonal (06-916; Upstate Biotechnology), anti-Ac-Lys-382 p53 rabbit polyclonal (01-010-179M; American Proteomics, Carlsbad, CA), anti-p300 mouse monoclonal (554215; BD Biosciences), anti-CBP rabbit polyclonal (SC-369; Santa Cruz Biotechnology), anti-HDAC1 mouse monoclonal (05-614; Upstate Biotechnology), anti-HDAC2 rabbit polyclonal (07-222; Upstate Biotechnology), anti-Pol II (H-224) rabbit polyclonal (SC-9001; Santa Cruz Biotechnology), anti-TBP mouse monoclonal (AB818; Abcam Inc., Cambridge, MA), or anti-TFIIH (Q-19) rabbit polyclonal (SC-292; Santa Cruz Biotechnology).

    Techniques: Expressing

    FIGURE 2. Association of transcription factors with Ac-Lys-373 p53 and Ac-Lys-382 p53. A, Western blot analysis of immunoaffinity-purified com- plexes eluted from anti-Ac-Lys-373 p53 and anti-Ac-Lys-382 p53 columns. B, association of transcription factors with acetylated isoforms of p53 after treatment with CG-1521 and TSA in combination for 8 h.

    Journal: Journal of Biological Chemistry

    Article Title: Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly

    doi: 10.1074/jbc.m609588200

    Figure Lengend Snippet: FIGURE 2. Association of transcription factors with Ac-Lys-373 p53 and Ac-Lys-382 p53. A, Western blot analysis of immunoaffinity-purified com- plexes eluted from anti-Ac-Lys-373 p53 and anti-Ac-Lys-382 p53 columns. B, association of transcription factors with acetylated isoforms of p53 after treatment with CG-1521 and TSA in combination for 8 h.

    Article Snippet: The antibodies used for the immunoprecipitation assay were: anti-total p53 mouse monoclonal (05-224; Upstate Biotechnology), antiAc-Lys-373 p53 rabbit polyclonal (06-916; Upstate Biotechnology), anti-Ac-Lys-382 p53 rabbit polyclonal (01-010-179M; American Proteomics, Carlsbad, CA), anti-p300 mouse monoclonal (554215; BD Biosciences), anti-CBP rabbit polyclonal (SC-369; Santa Cruz Biotechnology), anti-HDAC1 mouse monoclonal (05-614; Upstate Biotechnology), anti-HDAC2 rabbit polyclonal (07-222; Upstate Biotechnology), anti-Pol II (H-224) rabbit polyclonal (SC-9001; Santa Cruz Biotechnology), anti-TBP mouse monoclonal (AB818; Abcam Inc., Cambridge, MA), or anti-TFIIH (Q-19) rabbit polyclonal (SC-292; Santa Cruz Biotechnology).

    Techniques: Western Blot, Purification

    FIGURE 3. Association of total and acetylated isoforms of p53 with p21 promoter after treatment with CG-1521 or TSA. A, schematic of the p21 promoter showing the 1.3 and 2.4 kb p53 response elements and control region at 5 kb. B, protein extracts from untreated () or CG-1521- and TSA-treated () LNCaP cells after 8 h of treatment were subjected to ChIP assays using antibodies against total p53, Ac-Lys-373 p53, and Ac-Lys-382 p53. Control immunopre- cipitations with normal IgGs were also performed (Mock).

    Journal: Journal of Biological Chemistry

    Article Title: Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly

    doi: 10.1074/jbc.m609588200

    Figure Lengend Snippet: FIGURE 3. Association of total and acetylated isoforms of p53 with p21 promoter after treatment with CG-1521 or TSA. A, schematic of the p21 promoter showing the 1.3 and 2.4 kb p53 response elements and control region at 5 kb. B, protein extracts from untreated () or CG-1521- and TSA-treated () LNCaP cells after 8 h of treatment were subjected to ChIP assays using antibodies against total p53, Ac-Lys-373 p53, and Ac-Lys-382 p53. Control immunopre- cipitations with normal IgGs were also performed (Mock).

    Article Snippet: The antibodies used for the immunoprecipitation assay were: anti-total p53 mouse monoclonal (05-224; Upstate Biotechnology), antiAc-Lys-373 p53 rabbit polyclonal (06-916; Upstate Biotechnology), anti-Ac-Lys-382 p53 rabbit polyclonal (01-010-179M; American Proteomics, Carlsbad, CA), anti-p300 mouse monoclonal (554215; BD Biosciences), anti-CBP rabbit polyclonal (SC-369; Santa Cruz Biotechnology), anti-HDAC1 mouse monoclonal (05-614; Upstate Biotechnology), anti-HDAC2 rabbit polyclonal (07-222; Upstate Biotechnology), anti-Pol II (H-224) rabbit polyclonal (SC-9001; Santa Cruz Biotechnology), anti-TBP mouse monoclonal (AB818; Abcam Inc., Cambridge, MA), or anti-TFIIH (Q-19) rabbit polyclonal (SC-292; Santa Cruz Biotechnology).

    Techniques: Control

    FIGURE 4. Association of transcription factors with p21 promoter after treatment with CG-1521 or TSA. A, schematic of the p21 promoter showing the TATA box, 1.3 and 2.4 kb p53 response elements, and control region at 5 kb. B, protein extracts from untreated () or CG-1521- and TSA-treated () LNCaPcellsafter8hoftreatmentweresubjectedtoChIPanalysisoftheproximalpromoterusingantibodiesagainstp300,CBP,HDAC1,HDAC2.C,ChIPanalysis of the basal promoter using antibodies against Pol II, TBP, and TFIIH. Control immunoprecipitations with normal IgGs were also performed (Mock).

    Journal: Journal of Biological Chemistry

    Article Title: Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly

    doi: 10.1074/jbc.m609588200

    Figure Lengend Snippet: FIGURE 4. Association of transcription factors with p21 promoter after treatment with CG-1521 or TSA. A, schematic of the p21 promoter showing the TATA box, 1.3 and 2.4 kb p53 response elements, and control region at 5 kb. B, protein extracts from untreated () or CG-1521- and TSA-treated () LNCaPcellsafter8hoftreatmentweresubjectedtoChIPanalysisoftheproximalpromoterusingantibodiesagainstp300,CBP,HDAC1,HDAC2.C,ChIPanalysis of the basal promoter using antibodies against Pol II, TBP, and TFIIH. Control immunoprecipitations with normal IgGs were also performed (Mock).

    Article Snippet: The antibodies used for the immunoprecipitation assay were: anti-total p53 mouse monoclonal (05-224; Upstate Biotechnology), antiAc-Lys-373 p53 rabbit polyclonal (06-916; Upstate Biotechnology), anti-Ac-Lys-382 p53 rabbit polyclonal (01-010-179M; American Proteomics, Carlsbad, CA), anti-p300 mouse monoclonal (554215; BD Biosciences), anti-CBP rabbit polyclonal (SC-369; Santa Cruz Biotechnology), anti-HDAC1 mouse monoclonal (05-614; Upstate Biotechnology), anti-HDAC2 rabbit polyclonal (07-222; Upstate Biotechnology), anti-Pol II (H-224) rabbit polyclonal (SC-9001; Santa Cruz Biotechnology), anti-TBP mouse monoclonal (AB818; Abcam Inc., Cambridge, MA), or anti-TFIIH (Q-19) rabbit polyclonal (SC-292; Santa Cruz Biotechnology).

    Techniques: Control

    FIGURE 5. Model of differential p21 promoter occupancy as a result of differential p53 acetylation. The p21 locus contains two distal p53 response elements at 2.4 and 1.3 upstream of the transcription start site. Priortotreatmentwithhistonedeacetylaseinhibitors,HDAC1andHDAC2arebothassociatedwiththep53REs. After treatment with CG-1521, the equilibrium shifts to a more active transcription complex, with both p53 binding sites at 2.4 and 1.3kb occupied by Ac-Lys-373 p53 (green), p300, and HDAC1 and the complex associating with TBP and TFIIH on the TATA box. In contrast, treatment with TSA shifts the equilibrium signif- icantly at 1.3 kb, which incorporates Ac-Lys-382 p53 (red) associated with CBP and HDAC2 and appears to be an inactive transcription complex, as the TATA box fails to associate with either TBP or TFIIH. The additional proteins shown to associate by co-immunoprecipitation, Ac-Lys-373 p53 or Ac-Lys-382, are shown in the inset.

    Journal: Journal of Biological Chemistry

    Article Title: Site-specific Acetylation of p53 Directs Selective Transcription Complex Assembly

    doi: 10.1074/jbc.m609588200

    Figure Lengend Snippet: FIGURE 5. Model of differential p21 promoter occupancy as a result of differential p53 acetylation. The p21 locus contains two distal p53 response elements at 2.4 and 1.3 upstream of the transcription start site. Priortotreatmentwithhistonedeacetylaseinhibitors,HDAC1andHDAC2arebothassociatedwiththep53REs. After treatment with CG-1521, the equilibrium shifts to a more active transcription complex, with both p53 binding sites at 2.4 and 1.3kb occupied by Ac-Lys-373 p53 (green), p300, and HDAC1 and the complex associating with TBP and TFIIH on the TATA box. In contrast, treatment with TSA shifts the equilibrium signif- icantly at 1.3 kb, which incorporates Ac-Lys-382 p53 (red) associated with CBP and HDAC2 and appears to be an inactive transcription complex, as the TATA box fails to associate with either TBP or TFIIH. The additional proteins shown to associate by co-immunoprecipitation, Ac-Lys-373 p53 or Ac-Lys-382, are shown in the inset.

    Article Snippet: The antibodies used for the immunoprecipitation assay were: anti-total p53 mouse monoclonal (05-224; Upstate Biotechnology), antiAc-Lys-373 p53 rabbit polyclonal (06-916; Upstate Biotechnology), anti-Ac-Lys-382 p53 rabbit polyclonal (01-010-179M; American Proteomics, Carlsbad, CA), anti-p300 mouse monoclonal (554215; BD Biosciences), anti-CBP rabbit polyclonal (SC-369; Santa Cruz Biotechnology), anti-HDAC1 mouse monoclonal (05-614; Upstate Biotechnology), anti-HDAC2 rabbit polyclonal (07-222; Upstate Biotechnology), anti-Pol II (H-224) rabbit polyclonal (SC-9001; Santa Cruz Biotechnology), anti-TBP mouse monoclonal (AB818; Abcam Inc., Cambridge, MA), or anti-TFIIH (Q-19) rabbit polyclonal (SC-292; Santa Cruz Biotechnology).

    Techniques: Binding Assay, Immunoprecipitation